Fluorescent Quality Assessment slide for structured illumination systems (SIM) 

Usage

Argolight slide are designed to routinely check the stability of the characteristics of your systems.

Description

Argo-SIM  slides are re-usable stable fluorescence slides for assessing and monitoring fluorescence-based imaging systems. The Argo-SIM slides are specifically designed for structured illumination microscopes, as well as any system using deconvolution algorithms.

Each Argo-SIM slide contains several fluorescent microscopic patterns designed to help the user detect aberration in imaging systems.

Their intensity and spectrum are stable for over 3 years.  The analysis of pattern images can be simplified using Argolight software solutions. More about slides stability

The slides are compatible with excitation from 350 nm to 650 nm (fluorescence excitation over 550 nm requires sensitive sensors).

In our group, we build custom microscopes and I found Argo-SIM very useful to check different aspects of the microscope performance at different wavelength. In particular the calibration in z-direction for accurate 3D measurement as well as the resolution measurement in image scanning microscopy was extremely useful. Takahiro Deguchi

Post Doctoral Researcher, IIT Central Research Lab Genova

Argolight slides come with Daybook 2
Argolight fluorescence properties

Ideal to:

  • Ensure reliability and stability of an imaging system – obtain coherent measurements comparable over several years (including fluorescence quantities).
  • Set-up and maintain quality management at a low cost for fluorescence measurements.

Argo-SIM strengths:

  • Simplicity: No need for refrigerated storage, no consumable to add.
  • Ruggedness: Withstands fall from 2m height thanks to its metallic enclosure.
  • Compatibility: Works with oil immersion, dry objectives and water immersion objectives (with the exception of water-dipping objectives)
  • Cost: Replaces consumable most tools and increases monitoring frequency.

Designed for high resolution systems:

Content of the slide

Each Argo-SIM slide contains 14 fluorescent patterns.

Argo-SIM pattern overview

Pattern Overview. This represents the overall repartition of patterns in the SIM slide with scale.

Argo-SIM pattern target

Pattern A – Target. Concentric circles with increasing radii from 10 μm to 120 μm with a step of 10 μm, featuring a target.

Argo-SIM pattern field of rings

Pattern B – 2D Matrix of rings. A 2D matrix of 21 × 21 rings, separated by 5 μm, on a total field of 110 μm × 110 μm. The field of rings is surrounded by eight landmarks, and exhibits a 3 μm long cross in its center.

Argolight 4x4 intensity pattern

Pattern C – 4 x 4 Intensity. Sixteen 6 μm-wide squares having different fluorescence intensity levels following a linear evolution, organized in a 4 × 4 matrix.

Argolight 2x16 intensity pattern

Pattern D – 2×16 Intensity Twice sixteen 15 μm × 0.7 μm rectangles having different fl uorescence intensity levels following a linear evolution, organized in a 2×16 matrix.

Argo-SIM Gradually spaced lines pattern

Pattern E – Gradually spaced lines Pairs of 36 μm-long lines which spacing gradually increases, from 0 to 390 nm, with a step of 30 nm. Four sets of lines are present: one vertical, one horizontal, and two oriented at + and – 45°

Pattern F – Matrix of crosses. A matrix of 4 × 4 crosses, having a length of 5 μm. The crosses are composed of vertical lines that are in the same plane, and by horizontal lines, going gradually deeper within the glass from 0 to 1.5 μm, with a step of 100 nm.

Argo-SIM 3D sphere pattern

Pattern G – Meridians of a sphere. Three circles of diameter 25 μm in different orthogonal planes, featuring the meridians of a sphere.

Argo-SIM repositionning crosses pattern

Pattern H – Repositioning crosses. The repositioning crosses are 20 μm long and are positioned 500 μm from one to another in the X direction, the Y direction, or both.

Argo-SIM 3D crossing stairs pattern

Pattern I – 3D Crossing stairs. Empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. There are four stairs in the slide, with varying steps: 1, 0.5, 0.25 and 0.125 μm.

Pattern J – Logo. Letters forming the name “Argolight”, and surrounded by a 80 μm × 18 μm frame.

Argo-SIM 3D Matrix of rings pattern

Pattern K – 3D Matrix of rings. A 3D matrix of 9 × 9 × 9 rings, separated by 5 μm, on a total volume of 40 μm × 40 μm × 40 μm.

Pattern L – Matrix of rings on a background. This pattern is identical to pattern A, with a background that is 10 μm below.

Pattern M – Geometrical figures. A circle, a triangle, a square, a pentagon, an hexagon, an heptagon, an octagon and a star with 16 arms.

Argo-SIM Grid pattern

Pattern N – Grid. A grid with a step of 10 μm, with 5 crosses of 5 μm length in some squares.

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