Measure microscopes performance & detect issues
You don’t know if you don’t measure it.
Troubleshooting microscopes or performing quality control starts with you having the relevant information. You need reliable information, quickly! so you can take the right action and save the situation.
But time-efficiency does not mean cutting back on information. We give you the best information/time ratio possible from a quick 5 minutes check or a 1 hour in-depth performance assessment.
Discover Argolight Troubleshooting and Quality Control software, DAYBOOK
Daybook works with Argolight hardware solutions
More than 10 automated quality tests, several tens of relevant metrics.
Field uniformity Test
In any fluorescence microscope, the spatial distribution of the illumination intensity is never constant. It is usually maximal in the center of the field of view (if the microscope is well-aligned), and decreases towards the edges, so that a biological sample is never homogeneously illuminated.
For information, other appellations than homogeneity can be found in the literature: uniformity, evenness, flatness of illumination, or shading. Here our approach is to use a matrix of fluorescent rings.
Lateral Resolution Test
The lateral resolution test is defined as the shortest distance between two lines on a fluorescent pattern that can still be distinguished by the observer or camera system as separate entities.
In any fluorescence microscope, a degradation of the lateral resolving power induces a loss of contrast in sample images. Many aspects can modify the system’s PSF and therefore its lateral resolving power.
Field Distortion Test
Field distortion is an optical aberration inducing image deformation, usually at the corners of the image. In the presence of distortion, the magnification is not constant over the FOV; it is dependent on the (x ; y) coordinates. In any fluorescence microscope, the knowledge of the field of view distortion is important when spatial information in an image, such as distances, is aimed to be measured.
Distortion results in spatial variation of the pixel size, i.e. the value of the pixel size depends on its location in the field of view. The distortion rate (DR) of microscope objectives is not provided by the manufacturers.
Intensity Response Test
The overall intensity response of fluorescence microscopes may evolve over time, especially on confocal microscopes, and widefield microscopes equipped with metal-halide illuminators. In order to check the linearity of the intensity response we are using sixteen squares having different fluorescence intensity levels following a linear evolution.
Line Spread Function Test
The Line Spread Function test provides information on how and how much the light spreads from a line. The intensity spreading from a line can be modelled with a Gaussian function, which full-width at half maximum (FWHM) informs on the spreading behavior.
The cross inside the field of rings can be used to extract the line spread function in both the vertical and horizontal directions.
Stage Drift During Z Stacking Test
The environmental conditions around a fluorescence microscope can influence its performances. This is even more true if high resolution and/or precision are required for a given experiment. In particular, the lateral stage drift during Z-stack imaging is important when temperature and air flow fluctuate.
Spectral Response Test
Fluorescence microscopes, although conceived preliminary to be imaging systems, can also acquire emission spectra, with a better spatial resolution but poorer spectral resolution than conventional spectrometers.
Correct interpretation of the emission spectra measured with a microscope requires the knowledge of its spectral response.
Lateral co-registration accuracy Test
In any fluorescence microscope, the knowledge of the chromatic shifts, both in the lateral and axial directions, is important when color information in an image is aimed to be used. In particular, for co-localization tests in images of biological samples stained with several labels, chromatic shifts between the different channels must be known and compensated to prevent from misinterpretation.
Stage Drift During Timelapse
When performing timelapse imaging, it is important to be sure that the lateral stage do not drift too much. This test allows to measure the lateral stage drift during a timelapse imaging, to ensure it is not too important for the application.
Stage Repositioning Repeatability Test
Stage repositioning repeatability is of importance for stitching imaging. This test allows to determine the stage repositioning repeatability after many round trips, to ensure if it is within the manufacturer specifications.
Set tolerance thresholds
You are not efficient when you spend time demonstrating that systems work well.
Quality thresholds are the solution. Show right away that systems are performing and focus on the real issues.
You can select one or two threshold values and the orientation of the traffic light colors.
Batch process images
Do you have several systems to assess? Stop wasting time when processing images one by one. Batch processing in Daybook analyzes multiple images at once.
Choose a naming convention, load your images and there you go!
Choose the right plan for you
Daybook comes in two plans. Choose the one that fits your needs.
- Run more than 11 automated analyses to measure field uniformity, chromatic aberrations (more here).
- Get relevant and comparable values.
- Load images from multiple format, including proprietary (*.CZI, *.LIF, *.ND2, *.IOR, etc).
- Export analysis report in PDF.
- Export raw data in open formats (.CSV or .TXT).
- Online support.
- Frequent auto-updates.
Quality ControlProactive approach
- All the features included in the Troubleshooting plan.
- Analyze a batch of images at once.
- Save results in an organized database.
- Track the performance of your system over time.
- Follow and monitor several systems with different configurations.
- Explore, share and report on the performance of your different system.
- Create your own tolerance thresholds to detect issues.