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Publications from Argolight

Find all of our different publications about Quality Control of fluorescence imaging systems.

Quality Control of Fluorescence Imaging Systems: A new tool for performance assessment and monitoring

Reference: A. Royon and N. Converset, “Quality Control of Fluorescence Imaging Systems: A new tool for performance assessment and monitoring,” Optik & Photonik 2, 22-25, DOI: 10.1002/opph.201700005 (April 2017).

Find it here: https://onlinelibrary.wiley.com/doi/abs/10.1002/opph.201700005

Keywords: Microscope quality control; Microscope performance assessment

Quality Control of Fluorescence Imaging Systems. A New Tool for Performance Assessment and Monitoring

Reference: A. Royon and N. Converset, “Quality Control of Fluorescence Imaging Systems. A New Tool for Performance Assessment and Monitoring,” Imaging & Microscopy (May 2016).

Find it here: https://www.imaging-git.com/applications/quality-control-fluorescence-imaging-systems

Keywords: Microscope quality control; Microscope performance assessment

Quantitative imaging and fluorescence microscopy: Towards quantitative fluorescence microscopy: A new solution for standardization, monitoring, and quality management

Reference: A. Royon, “Quantitative imaging and fluorescence microscopy: Towards quantitative fluorescence microscopy: A new solution for standardization, monitoring, and quality management,” Laser Focus World (June 2015).

Find it here: https://www.laserfocusworld.com/articles/print/volume-51/issue-06/biooptics-world/biooptics-features/quantitative-imaging-and-fluorescence-microscopy-towards-quantitative-fluorescence-microscopy-a-new-solution-for-standardization-monitoring-and-quality-management.html

Keywords: Quantitative imaging

Calibration of Fluorescence Microscopes – A New Durable Multi-Dimensional Ruler

Reference: A. Royon and G. Papon, “Calibration of Fluorescence Microscopes – A New Durable Multi-Dimensional Ruler,” Imaging and Microscopy (August-September 2013).

Find it here: https://www.imaging-git.com/applications/calibration-fluorescence-microscopes

Keywords: Microscope calibration

Fluoreszierende sub-Mikrometer-Strukturen für die Kalibrierung und Justage von Mikroskopen

Reference: A. Royon and G. Papon, “Fluoreszierende sub-Mikrometer-Strukturen für die Kalibrierung und Justage von Mikroskopen,” Biophotonik 1 (February 2013).

Find it here: https://www.photonik.de/fluoreszierende-sub-mikrometer-strukturen-fuer-die-kalibrierung-und-justage-von-mikroskopen/150/21370/274356

Keywords: Microscope calibration

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Publications of work using Argolight products

Find all of our different publications and their work done with Argolight products.

Best practices and tools for reporting reproducible fluorescence microscopy methods

Product: Argo-SIM

Reference: P. Montero Llopis, R. A. Senft, T. J. Ross-Elliott, R. Stephansky, D. P. Keeley, P. Koshar, G. Marqués, Y.-S. Gao, B. R. Carlson, T. Pengo, M. A. Sanders, L. A. Cameron, and M. S. Itano, “Best practices and tools for reporting reproducible fluorescence microscopy methods,” Nature Methods 18, DOI: 10.1038/s41592-021-01156-w (June 2021).

Affiliation:
– MicRoN Core, Harvard Medical School (Boston, USA)
– Department of Genetics, Blavatnik Institute, Harvard Medical School (Boston, USA)
– Neuroscience Microscopy Core, University of North Carolina (Chapel Hill, USA)
– University Imaging Centers and Department of Neuroscience, University of Minnesota (Minneapolis, USA)
– Duke Light Microscopy Core Facility, Duke University (Durham, USA)
– University of Minnesota Informatics Institute, University of Minnesota (Minneapolis, USA)

They cited us within the framework of a study aiming to guide and assist researchers in writing rigorous and reproducible microscopy methods, by proposing minimal guidelines to ensure rigor and reproducibility in fluorescence light microscopy. The sphere, the gradually spaced lines and the 4×4 intensity gradation patterns of an Argo-SIM slide were used to straightforwardly show how the chromatic aberration, the optical resolution and detector sensitivity influence the quality of fluorescence microscopy images.

Citation page 6 & 7:

A 3D rendering of the 3D sphere pattern on the ArgoLight-SIM calibration slide acquired with two channels (488 nm, green; 561 nm, magenta) using either an Olympus Plan Apo 40×/1.3 NA DIC (left) or an Olympus Plan Fluor 40×/1.3 NA DIC (center) objective. […] Fluorescence images of a pattern consisting of lines with incrementally increasing spacing on the ArgoLight-SIM calibration slide acquired with the same objective and a camera with either a 6.5-μm (left) or 16-μm (right) photodiode size. […] Fluorescence images of a pattern consisting of a repeating series of lines with progressively decreasing intensity (pattern C) on the ArgoLight-SIM calibration slide were acquired with an Andor Zyla sCMOS 4.2 plus camera under low-light conditions (left panels) or high-light conditions (right panels).

Source link: https://www.nature.com/articles/s41592-021-01156-w

Keywords: Quality, rigor and reproducibility

Super-resolved live-cell imaging using random illumination microscopy

Product: Argo-SIM

Reference: T. Mangeat, S. Labouesse, M. Allain, A. Negash, E. Martin, A. Guénolé, R. Poincloux, C. Estibal, A. Bouissou, S. Cantaloube, E. Vega, T. Li, C. Rouvière, S. Allart, D. Keller, V. Debarnot, X. B. Wang, G. Michaux, M. Pinot, R. Le Borgne, S. Tournier, M. Suzanne, J. Idier, and A Sentenac, “Super-resolved live-cell imaging using random illumination microscopy,” Cell Reports Methods 1, DOI: 10.1016/j.crmeth.2021.100009, 1-15 (May 2021).

Affiliation:
– LITC Core Facility, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS (Toulouse, France)
– Institut Fresnel, Aix Marseille Université, CNRS, Centrale Marseille (Marseille, France)
– Molecular, Cellular & Developmental Biology (MCD), Center of Integrative Biology (CBI), Toulouse University, CNRS, UPS (Toulouse, France)
– Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS (Toulouse, France)
– INSERM Université de Toulouse, UPS, CNRS, Centre de Physiopathologie de Toulouse Purpan (CPTP) (Toulouse, France)
– Université de Rennes, CNRS, Institut de Génétique et Développement de Rennes (IGDR) – UMR 6290 (Rennes, France)
– Laboratoire des Sciences du Numérique de Nantes, CNRS UMR 6004 (Nantes, France)

They cited us within the framework of a study dealing with a new, simple and low-cost super-resolution microscopy method, based on speckled illumination, called random illumination microscopy (RIM), that matches traditional structured illumination microscopy (SIM) performances in a more robust fashion. The gradually spaced lines of an Argo-SIM slide were employed as ground truths to demonstrate the resolution performance of this method.

Citation page 4:

We estimated the transverse resolution of RIM by using a resolution target (Argo-SIM slide, Argolight) compared with widefield microscopy (Figure 1C). Under optimal conditions, with a 1.49 numerical aperture (NA) objective and an excitation wavelength of 405 nm, RIM achieved a sub-100-nm resolution of 76 nm, as estimated by Fourier image resolution (FIRE), comparable with the best 2D SIM resolution of 84 nm.

Source link: https://www.sciencedirect.com/science/article/pii/S2667237521000096

Keywords: Resolution

The wavelet-based denoising of images in Fiji, with example applications in structured illumination microscopy


Product: Argo-SIM

Reference: M. Capek, M. Blazikova, I. Novotny, H. Chmelova, D. Svoboda, B. Radochova, J. Janacek, and O. Horvath, “The wavelet-based denoising of images in Fiji, with example applications in structured illumination microscopy,” Image Analysis & Stereology 40, DOI: 105566/ias.2432, 3-16 (April 2021).

Affiliation:
– Institute of Molecular Genetics of the Czech Academy of Sciences, Light Microscopy Core Facility (Prague, Czech Republic)
– Institute of Physiology of the Czech Academy of Sciences, Laboratory of Biomathematics (Prague, Czech Republic)
– Masaryk University, Faculty of Informatics, Centre for Biomedical Image Analysis (Brno, Czech Republic)

They cited us within the framework of a study dealing with a new method allowing to filter images captured with super-resolution structured illumination microscopy (SIM). The proposed method uses discrete wavelet transform, instead of standard filtration techniques (such as convolution- or Fourier transform-based methods). The 2×16 intensity gradation of an Argo-SIM slide was employed as a ground truth to evaluate the fidelity of the discrete wavelet-based denoising method.

Citation page 7:

An example of 2D DWT is given in Fig. 6 which shows the original image, structures acquired by a Leica SP8 confocal microscope from an Argolight confocal calibration slide, as well as the transformed image.

Source link: https://www.ias-iss.org/ojs/IAS/article/view/2432/1140

Keywords: Filtration in SIM

High-fidelity structured illumination microscopy by point-spread-function engineering

Product: Argo-SIM

Reference: G. Wen, S. Li, L. Wang, X. Chen, Z. Sun, Y. Liang, X. Jin, Y. Xing, Y. Jiu, Y. Tang, and H. Li, “High-fidelity structured illumination microscopy by point-spread-function engineering” Light: Science & Applications 10, DOI: 10.1038/s41377-021-00513-w, 1-12 (April 2021).

Affiliation:
– Jiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences (Suzhou, China)
– Academy for Engineering and Technology, Fudan University (Shanghai, China)

They cited us within the framework of a study dealing with a new high-fidelity reconstruction algorithm for super-resolution structured illumination microscopy (SIM). The star and the 2D matrix of rings of an Argo-SIM slide were employed as ground truths to quantitatively evaluate the fidelity of the reconstruction algorithms.

Citation page 8:

Fluorescent beads of 100-nm diameter and commercial Argo-SIM slide were employed as standard samples to quantitatively evaluate the fidelity of reconstruction algorithms.

Source link: https://www.nature.com/articles/s41377-021-00513-w

Keywords: Reconstruction in SIM

Unified joint reconstruction approach for random illumination microscopy

Product: Argo-SIM

Reference: P. Liu, “Unified joint reconstruction approach for random illumination microscopy” Biomedical Optics Express 11, 5147-5165, DOI: 10.1364/BOE.399547 (June 2020).

Affiliation:
– College of Optics and Electronic Technology, China Jiliang University (Hangzhou, China)
– Laboratoire des Sciences du Numérique de Nantes, Ecole Centrale de Nantes (Nantes, France)

He cited us within the framework of a study dealing with a unified joint reconstruction approach in random illumination microscopy (RIM) using uncontrolled speckle patterns. This would provide the possibility to design inexpensive and versatile structured illumination microscopy (SIM) devices. The gradually spaced lines pattern of an Argo-SIM slide was used to reveal the superiority in terms of resolution of the RIM technique compared to conventional SIM.

Citation page 3:

The Line section plot extracted from Argolight reconstructions […] reveals that RIM is superior in resolution.

Source link: https://www.osapublishing.org/boe/fulltext.cfm?uri=boe-11-9-5147&id=434562

Keywords: Resolution

ℓ1-regularized maximum likelihood estimation with focused-spot illumination quadruples the diffraction-limited resolution in fluorescence microscopy


Product: Argo-SIM

Reference: J. Xing, S. Chen, S. Becker, J.-Y. Yu, and C. Cogswell, “ℓ1-regularized maximum likelihood estimation with focused-spot illumination quadruples the diffraction-limited resolution in fluorescence microscopy,” Optics Express 28, 39413-39429, DOI: 10.1364/OE.41157 (December 2020).

Affiliation:
– Department of Electrical, Computer, and Energy Engineering, University of Colorado (Boulder, USA)
– Department of Applied Mathematics, University of Colorado (Boulder, USA)

They cited us within the framework of a study dealing with the improvement of the accuracy of a computational super-resolution approach [the non-negative least squares (NNLS) method]. The gradually spaced lines pattern of an Argo-SIM slide was used to show the resolution enhancement brought by the improvement of the NNLS approach.

Citation page 39424:

To verify these findings in a real-world imaging system, we performed experiments imaging a fluorescent, resolution-test sample made by Argolight. This sample contains successive fluorescently-labeled line pairs separated by different distances ranging for 0nm (no separation) to 270nm.

Source link: https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-28-26-39413&id=444754

Keywords: Resolution

Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy


Product: Argo-SIM

Reference: S. Qin, S. Isbaner, I. Gregor, and J. Enderlein, “Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy,” Nature Protocols 16, 164-181, DOI: 10.1038/s41596-020-00408-x (November 2020).

Affiliation:
– Department of Physics, Third Institute of Physics-Biophysics, Georg August University (Göttingen, Germany)
– Cluster of Excellence ‘Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells’ (MBExC), Georg August University (Göttingen, Germany)

They cited us within the framework of a study dealing with the development of a confocal spinning-disk microscope using image scanning microscopy. The gradually spaced lines pattern of an Argo-SIM slide was used to demonstrate the resolution enhancement brought by this instrument.

Citation page 16:

Figure 4c,d shows a pattern of gradually spaced lines of the Argo-SIM slide.

Source link: https://www.nature.com/articles/s41596-020-00408-x

Keywords: Resolution

Micro-stepping Extended Focus reduces photobleaching and preserves structured illumination super-resolution features


Product: Argo-SIM

Reference: X. Hu, S. Jalal, M. Sheetz, O. Bakke, and F. Margadant, “Micro-stepping Extended Focus reduces photobleaching and preserves structured illumination super-resolution features,” Journal of Cell Science 133, 1-12, DOI:10.1242/jcs.240796 (April 2020).

Affiliation:
– Department of Biosciences, University of Oslo, Blindern (Oslo, Norway)
– Mechanobiology Institute, National University of Singapore (Singapore)
– Department of Biological Sciences, Columbia University (New York, USA)
– Department of Biochemistry and Molecular Biology, University of Texas Medical Branch (Galveston, USA)
– Department of Biomedical Engineering, National University of Singapore (Singapore)

They cited us within the framework of a study dealing with an upgrade to spinning disk confocal microscopes that dramatically improves photon balance and imaging speed for various imaging applications in projections while simultaneously preserving super-resolution features. For this purpose, 3D patterns of the Argo-SIM

Citation page 3:

To validate the precision control of projection depth of EF, we performed several experiments with the Argolight SIM test slides. The Argolight test slides series contains optical band gaps that mimic the behavior of fluorophores. Each test slide contains several known geometrical structures that are rendered very precisely. It is commonly used by optical microscopists to evaluate various performance parameters of the systems of interest, such as illumination homogeneity and the resolution limit. Patterns I and G of the Argolight SIM slide were chosen as quantitative tests for the depth resolution because they feature high-resolution structures in the Z-direction.

Source link: https://jcs.biologists.org/content/early/2020/04/01/jcs.240796

Keywords: Microscope performance validation

Which elements to build co-localization workflows? From metrology to analysis


Product: Argo-HM

Reference: P. Mascalchi and F. P. Cordelières, “Which elements to build co-localization workflows? From metrology to analysis,” In: Rebollo E., Bosch M. (eds) Computer Optimized Microscopy. Methods in Molecular Biology 2040, Humana, New York, NY, DOI: 10.1007/978-1-4939-9686-5_10 (August 2019).

Affiliation: Bordeaux Imaging Center (Bordeaux, France)

They cited us within the framework of a review on elementary bricks and methods required to build co-localization workflows. One step consists in assessing the co-registration inaccuracy between channels, which originates from the fluorescence microscope. For this purpose, the field of rings pattern of an Argo-HM slide was used to measure the lateral shifts between two channels in the entire field of view.

Citation page 188:

Checking the co-registration requires a sample where dyes of similar nature as the actual samples are known to be co-registered. Two options are available: using reference slides (e.g., Argolight™ slides used in Fig. 4) or freshly prepared fluorescent bead slides.

Source link: https://link.springer.com/protocol/10.1007/978-1-4939-9686-5_10

Keywords: Channel co-registration

High-speed imaging of scattering particles flowing through turbid media with confocally aligned, oblique plane illumination


Product: Argo-HM

Reference: G. N. McKay, A. Y. Trick, and N. J. Durr, “High-speed imaging of scattering particles flowing through turbid media with confocally aligned, oblique plane illumination,” Proceedings of SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108902K, DOI: 10.1117/12.2509865 (March 2019).

Affiliation: Johns Hopkins University (Baltimore, USA)

They cited us within the framework of a study dealing with high-speed imaging of scattering particles flowing through turbid media with confocally-aligned, oblique plane illumination. The alignment verification of the described light-sheet based microscopy imaging system (SCAPE = swept-confocally aligned planar excitation) was performed using the 3D crossing stairs pattern of an Argo-HM slide as a ground truth.

Citation page 4:

Alignment of the system was verified using 3D volumetric SCAPE scans in a modified fluorescence mode, where a fluorescence emission filter was added to infinite conjugate space. The first target was a DTDCI-soaked lens cleaning fiber in methanol, and the second target was the 3D Crossing Stairs fluorescent target of an Argolight Argo-HM slide using an added 488nm illumination line.

Source link: https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10890/108902K/High-speed-imaging-of-scattering-particles-flowing-through-turbid-media/10.1117/12.2509865.short?SSO=1

Keywords: Microscope alignment

Successful optimization of reconstruction parameters in structured illumination microscopy – a practical guide


Product: Argo-SIM

Reference: C. Karras, M. Smedh, R. Förster, H. Deschout, J. Fernandez-Rodriguez, and R. Heintzmann, “Successful optimization of reconstruction parameters in structured illumination microscopy – a practical guide,” Optics Communication 436, 69-75, DOI: 10.1016/j.optcom.2018.12.005 (December 2018).

Affiliation:
– Leibniz Institute of Photonic Technology (IPHT) (Jena, Germany)
– Centre of Cellular Imaging, Core Facilities, the Sahlgrenska Academy, University of Gothenburg (Gothenburg, Sweden)
– Institute of Physical Chemistry, Friedrich-Schiller-University (FSU) (Jena, Germany)

They cited us within the framework of a study dealing with the impact of different reconstruction parameters in super-resolution structured illumination microscopy (SIM) on image artifacts. For this purpose, the gradually spaced lines pattern of an Argo-SIM slide was used to characterize the artifact classes and their correlation with the image spectra as well as the reconstruction parameters.

Citation page 1:

Two different samples were used in order to study the impact of reconstruction parameters on the post-processing of structured illumination images: (i) A photostable commercial sample (Argo-SIM slide, Argolight, France) consisting of fluorescing double line pairs (spacing from 150 nm to 240 nm, λex = 360–550 nm, line thickness below 100 nm).

Source link: https://www.sciencedirect.com/science/article/pii/S003040181831054X?via%3Dihub

Keywords: Reconstruction in SIM

Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy


Product: Argo-HM

Reference: Y. Chen, W. Liu, Z. Zhang, C. Zheng, Y. Huang, R. Cao, D. Zhu, L. Xu, M. Zhang, Y.-H. Zhang, J. Fan, L. Jin, Y. Xu, C. Kuang, and X. Liu, “Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy,” DOI: 10.1038/s41467-018-07244-4, Nature Communications 9:4818, 1-8 (November 2018).

Affiliation:
– State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University (Hangzhou, China)
– Shanxi Provincial Key Laboratory for Biomedical Imaging and Big Data, North University of China (Taiyuan, China)
– Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology (Wuhan, China)
– Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University (Hangzhou, China)
– Collaborative Innovation Center of Extreme Optics, Shanxi University (Taiyuan, China)

They cited us within the framework of a study dealing with the development of a new multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. The gradually spaced lines pattern of an Argo-HM slide was used as a ground truth sample to validate the performance of multi-angle interference microscopy (MAIM) in terms of resolution.

Citation page 2:

We used gradually spaced lines (Argo-HM) and silica microsphere (Bangs Laboratories, Inc.) ground-truth samples to experimentally validate MAIM. The lateral resolution enhancement was confirmed by separating 100-nm distance in the spaced line sample.

Source link: https://www.nature.com/articles/s41467-018-07244-4

Keywords: Resolution

A unified joint reconstruction approach in structured illumination microscopy using unknown speckle patterns


Product: Argo-SIM

Reference: P. Liu, “A unified joint reconstruction approach in structured illumination microscopy using unknown speckle patterns,” hosted on arXiv:1811.00283 [eess.IV] (November 2018).

Affiliation:
– Laboratoire des Sciences du Numérique de Nantes (LS2N), Ecole Centrale de Nantes (Nantes, France)

He cited us within the framework of a study dealing with a unified joint reconstruction approach in structured illumination microscopy (SIM) using unknown speckle patterns (blind-speckleSIM). The gradually spaced lines pattern of an Argo-SIM slide was used to reveal the superiority in terms of resolution of the blind-speckleSIM technique compared to conventional SIM.

Citation page 7:

Line section plot of Argolight reconstructions in Fig. 11 reveals that blind-speckleSIM is superior in resolution.

Source link: https://arxiv.org/pdf/1811.00283.pdf

Keywords: Reconstruction in SIM

Remote refocus enables class-leading spatiotemporal resolution in 4D optical microscopy


Product: Argo-SIM

Reference: A. Millett-Sikking, N. H. Thayer, A. Bohnert, and A. G. York, “Remote refocus enables class-leading spatiotemporal resolution in 4D optical microscopy,” hosted on GitHub Pages, DOI:10.5281/zenodo.1146083 (January 2018).

Affiliation:
– Calico Life Sciences LLC (San Francisco, USA)

They cited us within the framework of a study dealing with the remote refocus technique. In the article, a modular high-performance design is provided to enable others to build their own. The concept, method, and rules of remote refocus are also presented to help others design their own. The target, the gradually spaced lines and the 3D matrix of rings patterns of an Argo-SIM slide were used to demonstrate the capability and performance of the provided remote refocus design.

Citation page 8:

We characterize image quality via Argolight’s SIM slide, with variable-spacing fluorescent features (down to 30 nm separation) which allow us to quickly and accurately measure spatial resolution over the full 3D field-of-view.

Source link: https://andrewgyork.github.io/remote_refocus/

Keywords: Microscope quality control

Exploring the Potential of Airyscan Microscopy for Live Cell Imaging


Product: Argo-SIM

Reference: K. Korobchevskaya, H. Colin-York, B. C. Lagerholm, and M. Fritzsche, “Exploring the Potential of Airyscan Microscopy for Live Cell Imaging,” Photonics 4, 41, DOI: 10.3390/photonics4030041 (July 2017).

Affiliation:
– Kennedy Institute for Rheumatology, University of Oxford (Oxford, UK)
– Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford (Oxford, UK)
– MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford (Oxford, UK)

They cited us within the framework of a study dealing with the exploration of the potential of Airyscan (multi-channel area detectors) microscopy for live cell imaging. In particular, the gradually spaced lines pattern of an Argo-SIM slide was used to measure BOTH the lateral resolution of the instrument and the signal-to-noise ratio (SNR) in the image. This approach allows the evaluation of a system’s performance in its whole within the well-known representation of the eternal triangle (speed vs resolution vs sensitivity).

Citation page 13:

To further illustrate these findings of the Argo-SIM slide, we performed a systematic quantitative analysis of the spatial resolution and the SNRs.

Source link: https://www.mdpi.com/2304-6732/4/3/41

Keywords: Resolution and SNR (Signal-to-noise ratio)

Hot Pixels Suppression in Structured Illumination Microscopy


Product: Argo-SIM

Reference: J. Pospisil, “Hot Pixels Suppression in Structured Illumination Microscopy,” in Proceedings of the International Student Scientific Conference Poster, ISBN 978-80-01-06153-4 (May 2017).

Affiliation:
– Multimedia Technology Group (MMTG) with department of Radioelectronics at FEE in CTU (Prague, Czech Republic)

He cited us within the framework of a study dealing with hot pixels suppression in structured illumination microscopy (SIM). The gradually spaced lines pattern of an Argo-SIM slide was used to demonstrate the performance of the presented hot pixel detection and suppression algorithm.

Citation page 2:

The Argolight test targets are designed for assessing the performances of fluorescence-based microscopy imaging systems.

Source link: http://radio.feld.cvut.cz/conf/poster/proceedings/Poster_2017/Section_EI/EI_041_Pospisil.pdf

Keywords: Algorithm performance assessment

Navigating challenges in the application of super-resolution microscopy


Product: Argo-SIM

Reference: T. J. Lambert and J. C. Waters, “Navigating challenges in the application of super-resolution microscopy,” Journal of Cell Biology, DOI: 10.1083/jcb.201610011 (December 2016).

Affiliation:
– Department of Cell Biology, Harvard Medical School (Boston, USA)

They cited us within the framework of a study dealing with the navigation of the challenges in the application of super-resolution microscopy, in particular, structured illumination microscopy (SIM). The gradually spaced lines pattern of an Argo-SIM slide was used to show how low signal-to-noise ratio acquisition conditions in SIM lead to reconstruction artifacts in the image.

Citation:

Optimization of super resolution microscopy is aided by standards for characterizing instrument performance and methods for assessing image quality. Useful standards include well-characterized biological structures (such as microtubules), nuclear pores, clathrin-coated pits, centrioles or synaptonemal complexes, DNA-origami rulers, and micropatterned slides (Argolight).

Source link: http://jcb.rupress.org/content/216/1/53

Keywords: Reconstruction in SIM

eSIP: A Novel Solution-Based Sectioned Image Property Approach for Microscope Calibration

Product: Argo-M

Reference: M. Butzlaff, A. Weigel, E. Ponimaskin, and A. Zeug, “eSIP: A Novel Solution-Based Sectioned Image Property Approach for Microscope Calibration,” PLOS ONE 10(8), DOI: 10.1371/journal.pone.0134980 (August 2015).

Affiliation:
– Cellular Neurophysiology, Center of Physiology, Hannover Medical School (Hannover, Germany)
– Carl Zeiss Microscopy GmbH (München, Germany)

They cited us within the framework of a study dealing with a novel solution-based sectioned image property (SIP) approach for microscope calibration. This article describes the solution-based eSIP method and shows result examples about the illumination inhomogeneity and axial resolution measurements. Besides, the grid of an Argo-M slide was used to measure the distortion of the field of view and the lateral co-registration inaccuracy.

Citation:

The so far only commercially available product which addresses to fulfil the requirements mentioned above (e.g., the production is standardized, and the long-term stability of fluorescence is guaranteed for five years) is the Argolight calibration slide. We therefore evaluated an ARGO-M slide (standard version of mid 2014) for its calibration properties and its usability for the eSIP layer approach.

Source link: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134980

Keywords: Field distortion; Channel co-registration

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Publications mentioning Argolight

Here some publications who are mentioning Argolight and our products.

Microscope calibration protocol for single-molecule microscopy

Reference: S. You, J. Chao, E. A. K. Cohen, E. S. Ward, and R. J. Ober, “Microscope calibration protocol for single-molecule microscopy,” Optics Express 29, 182-207, DOI: 10.1364/OE.408361 (January 2021).

Affiliation:
– Department of Biomedical Engineering, Texas A&M University (College Station, USA)
– Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center (College Station, USA)
– Astero Technologies LLC (College Station, USA)
– Department of Mathematics, Imperial College London (London, UK)
– Centre for Cancer Immunology, Faculty of Medicine, University of Southampton (Southampton, UK)

They mentioned us within the framework of a study dealing with the calibration of single-molecule microscopes at the nanometer scale.

Citation page 183:

To overcome the various limitations of approaches that use beads or DNA origami, in recent years techniques have been developed to create slides imprinted with defined patterns for system calibration. For example, Argolight uses a laser to induce into glass substrates fluorescent materials that are stable and have a broadband emission spectrum. An example of an Argolight slide contains different fluorescent patterns in two and three dimensions, the elementary structure of which is an empty cylinder with a diameter of about 0.7 μm.

Source link: https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-29-1-182&id=445045

Keywords: Microscope calibration

Oriented attachment and activated dipoles leading to anisotropic H-bond-free self-assembly of n-acene derivatives into organic nanoribbons emitting linearly polarised blue light

Product: Argo-U

Reference: P. Schäfer, L. Gartzia-Rivero, M.-T. Kao, C. Schäfer, S. Massip, C. de Vet, G. Raffy, and A. Del Guerzo, “Oriented attachment and activated dipoles leading to anisotropic H-bond-free self-assembly of n-acene derivatives into organic nanoribbons emitting linearly polarised blue light,” Journal of Materials Chemistry C 10, 1-14, DOI: 10.1534/g3.120.401465 (October 2020).

Affiliation:
– University of Bordeaux, CNRS, Bordeaux INP, Institut des Sciences Moléculaires (Talence, France)
– Department of Physical Chemistry, University of the Basque Country (UPV/EHU) (Bilbao, Spain)
– IECB, University of Bordeaux, CNRS UMS3033, INSERM US001 (Pessac, France)

They mentioned us within the framework of a study dealing with the design of self-assembled nanoobjects with very high aspect ratios. They used an Argo-U slide to correct the spectral intensity response of their spectral confocal microscope.

Citation page 10:

Spectral intensity correction was performed with an ARGO-U SLB8 slide (Argolight).

Source link: https://pubs.rsc.org/en/content/articlelanding/2020/tc/d0tc04789a#!divAbstract

Keywords: Spectral intensity correction

Preventing photomorbidity in long-term multi-color fluorescence imaging of S. cerevisiae and S. pombe

Product: Argo-M

Reference: Gregor W. Schmidt, Andreas P. Cuny, and Fabian Rudolf, “Preventing photomorbidity in long-term multi-color fluorescence imaging of S. cerevisiae and S. pombe,” G3: Genes-Genomes-Genetics 10, 1-14, DOI: 10.1534/g3.120.401465 (October 2020).

Affiliation:
– ETH Zurich, Department of Biosystems Science and Engineering (Basel, Switzerland)

They mentioned us within the framework of a study presenting guidelines to avoid the confounding effects of excitation light in multi-color long-term imaging. They measured the effect of the administered excitation light on growth rate, so-called photomorbidity, in yeast. They used an Argo-M slide to measure the light intensity of the excitation light of their epifluorescence microscope.

Citation page 25:

In short, the field diaphragm of the epifluorescence light path was adjusted such that the diaphragm was visible in the field of view of the camera, using the 40x Plan Fluor Oil DIC N2 NA 1.3 objective (MRH01401, Nikon Instruments AG, Egg, Switzerland) and a fluorescent Argo-M Standard microscopy slide (Argolight, Talence, France).

Source link: https://www.g3journal.org/content/early/2020/10/06/g3.120.401465.abstract

Keywords: Microscope calibration

Revealing architectural order with quantitative label-free imaging and deep learning

Product: Argo-SIM

Reference: S.-M. Guo, L.-H. Yeh, J. Folkesson, I. E. Ivanov, A. P. Krishnan, M. G. Keefe, E. Hashemi, D. Shin, B. B. Chhun, N. H. Cho, M. D. Leonetti, M. H. Han, T. J. Nowakowski, and S. B. Mehta, “Revealing architectural order with quantitative label-free imaging and deep learning,” eLife 9:e55502, 1-33, DOI: 10.7554/eLife.55502 (July 2020).

Affiliation:
– Chan Zuckerberg Biohub (San Francisco, USA)

They mentioned us within the framework of a study dealing with the synergistic use of polarized light microscopy, reconstruction of complementary optical properties, and deep neural networks to identify ordered structures in living systems. The 3D matrix of rings of an Argo-SIM slide was imaged to compute transformation matrices, used to register volumes of 3D kidney and brain tissues.

Citation page 25:

We multiplexed the acquisition of label-free and fluorescence volumes. The volumes were registered using transformation matrices computed from similarly acquired multiplexed volumes of 3D matrix of rings from the ARGO-SIM test target (Argolight).

Source link: https://elifesciences.org/articles/55502

Keywords: Volume registration

Fluorescence calibration standards made from broadband emitters encapsulated in polymer beads for fluorescence microscopy and flow cytometry

Reference: K. Hoffmann, N. Nirmalananthan-Budau, and U. Resch-Genger, “Fluorescence calibration standards made from broadband emitters encapsulated in polymer beads for fluorescence microscopy and flow cytometry,” Analytical and Bioanalytical Chemistry, DOI: 10.1007/s00216-020-02664-y (May 2020).

Affiliation:
– BAM Federal Institute for Materials Research and Testing (Berlin, Germany)

They mentioned us within the framework of a study dealing with the design and characterization of a set of spectral calibration beads, intended for the determination and regular control of the spectral characteristics of fluorescence microscopes and other fluorescence measuring devices for the readout of bead-based assays.

Citation:

Also for fluorescence microscopy, in addition to a very small number of relatively expensive structured calibration tools like the slide from Argolight, different calibration beads have been commercialized to determine parameters like resolution x/y/ z, intensity calibration, color adjustment, instrument alignment, and stability.

Source link: https://link.springer.com/article/10.1007/s00216-020-02664-y

Keywords: Microscope calibration

Fluorescence Correlation Methods for Determining Absolute Numbers of Molecules from Microscopy Images

Reference: A. Sasaki, M. Halter, J. T. Elliott, “Fluorescence Correlation Methods for Determining Absolute Numbers of Molecules from Microscopy Images,” J-STAGE, DOI: 10.11169/bioimages.27.13 (May 2019).

Affiliation:
– Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) (Tsukuba, Japan)
– Cell Systems Science Group, Biosystems and Biomaterials Division, Material Measurement Laboratory, National Institute of Standards and Technology (Gaithersburg, USA)

They mentioned us within the framework of a review presenting an overview of the usefulness and current limitations of the FCS technique for quantitative confocal fluorescence imaging and its standardization by evaluating the microscopes system performance in collecting relevant image data.

Citation page 19:

Calibration slides with fluorescent patterns were recently developed. These slides are suitable for calibrating lateral chromatic shifts, system intensity response, illumination homogeneity and spatial resolution.

Source link: https://www.jstage.jst.go.jp/article/bioimages/27/0/27_13/_article/-char/en

Keywords: Benchmarking fluorescence microscope

Detection and quantification of RNA decay intermediates using XRN1-resistant reporter transcripts

Product: Argo-HM

Reference: F. Voigt, J. V. Gerbracht, V. Boehm, I. Horvathova, J. Eglinger, J. A. Chao and N. H. Gehring, “Detection and quantification of RNA decay intermediates using XRN1-resistant reporter transcripts,” DOI: 10.1038/s41596-019-0152-8, Nature Protocols 14, 1603-1633 (April 2019).

Affiliation:
– Friedrich Miescher Institute for Biomedical Research (Basel, Switzerland)
– Institute for Genetics, University of Cologne (Cologne, Germany)
– University of Basel (Basel, Switzerland)

They mentioned us for multicolor channel alignment on a multipoint confocal spinning disk microscope within the framework of RNA decay intermediates detection and quantification.

Citation page 1613:

Multicolor calibration slide for channel alignment (Argolight, cat. no. Argo-SLF-001).

Source link: https://www.nature.com/articles/s41596-019-0152-8

Keywords: Channel co-registration

Using the NoiSee workflow to measure signal-to-noise ratios of confocal microscopes

Reference: A. Ferrand, K. D. Schleicher, N. Ehrenfeuchter, W. Heusermann and O. Biehlmaier, “Using the NoiSee workflow to measure signal-to-noise ratios of confocal microscopes,” DOI: 10.1038/s41598-018-37781-3, Nature Scientific Reports 9, Article number 1165 (February 2019).

Affiliation:
– Imaging Core Facility, Biozentrum, University of Basel (Basel, Switzerland)

They mentioned us for resolution measurement within the framework of a study about signal-to-noise ratio assessment of confocal microscopes performance.

Citation page 11:

[…], GATTAquant nanorulers or Argolight slides, with their associated software are useful tools to address resolution.

Source link: https://www.nature.com/articles/s41598-018-37781-3

Keywords: Resolution and SNR (Signal-to-Noise Ratio)

Live Imaging of mRNA Transcription in Drosophila Embryos

Product: Argo-HM

Reference: C. A. Perez-Romero, H. Tran, M. Coppey, A. M. Walczak, C. Fradin, and N. Dostatni, “Live Imaging of mRNA Transcription in Drosophila Embryos,” DOI: 10.1007/978-1-4939-8772-6_10, In: Dubrulle J. (eds) Morphogen Gradients – Methods in Molecular Biology 1863, Humana Press, New York, NY (October 2018).

Affiliation:
– Institut Curie, PSL Research University, CNRS, Sorbonne Université, Nuclear Dynamics (Paris, France)
– McMaster University (Hamilton, Canada)
– Ecole Normale Supérieure, PSL Research University, CNRS, Sorbonne Université, Physique Théorique (Paris, France)
Institut Curie, PSL Research University, CNRS, Sorbonne Université, Physico Chimie (Paris, France)

They mentioned us as one of the possible solutions to control the performance of a confocal microscope to achieve quantitative imaging.

Citation page 179:

Commercial fluorescence standards such as Argolight (Argo-HM) that work for most wavelengths and are photostable for years are also available, but at a cost.

Source link: https://link.springer.com/protocol/10.1007/978-1-4939-8772-6_10

Keywords: Quantitative imaging

Detection of the First Round of Translation: The TRICK Assay

Reference: F. Voigt., J. Eglinger., J.A. Chao, “Detection of the First Round of Translation: The TRICK Assay,” DOI: 10.1007/978-1-4939-7213-5_25, In: Gaspar I. (eds) RNA Detection. Methods in Molecular Biology, vol 1649. Humana Press, New York, NY (February 2018).

Affiliation:
– Friedrich Miescher Institute for Biomedical Research (Basel, Switzerland)

They mentioned us for multicolor channel alignment on a multipoint confocal spinning disk microscope within the framework of RNA imaging acquisition protocol.

Citation page 376:

Multicolor calibration slide for channel alignment (e.g., Argolight, type SLF-001).

Source link: https://link.springer.com/protocol/10.1007/978-1-4939-7213-5_25

Keywords: Channel co-registration

Microscope calibration using laser written fluorescence

Reference: A. D. Corbett, M. Shaw, A. Yacoot, A. Jefferson, L. Schermelleh, T. Wilson, M. Booth, and P. S. Salter, “Microscope calibration using laser written fluorescence,” DOI: 10.1364/OE.26.021887, Optics Express 26, 21887-21900 (August 2018).

Affiliation:
– Department of Physics and Astronomy, University of Exeter (Exeter, UK)
– National Physical Laboratory (Teddington, UK)
– Micron Oxford Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford (Oxford, UK)
– Department of Engineering Science, University of Oxford (Oxford, UK)
– Department of Computer Science, University College London (London, UK)

They mentioned us as one of the main instigators in the field of calibration of fluorescence microscopes.

Citation page 21888:

Argolight use a laser to write features by the coalescing of metallic nanoparticles distributed throughout a glass substrate. The features are stable with a broadband emission spectrum.

Source link: https://www.osapublishing.org/oe/abstract.cfm?uri=oe-26-17-21887

Keywords: Microscope calibration

Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis

Reference: J. Bienzeisler, M. Landry, A. Llorente and G. Hüttmann, “Analysis of the in-vivo GABAB receptor relocalization and oligomerization in chronic pain conditions using spatial intensity distribution analysis,” DOI: 10.1515/cdbme-2017-0141, Current Directions in Biomedical Engineering 3, 669-673 (September 2017).

Affiliation:
– Universität zu Lübeck, Medizinische Ingenieurwissenschaft (Lübeck, Germany)
– Université de Bordeaux, IINS (Bordeaux, France)
– Universidad del País Vasco (Bilbao, Spain)
– Universität zu Lübeck, Institut für Biomedizinische Optik (Lübeck, Germany)

They mentioned us for intensity response linearity assessment within the framework of a study about the quantification of the oligomerization and density of proteins in images from a confocal fluorescence microscope.

Citation page 670:

The slope of the linear correlation between the mean pixel value and the respective variance was asserted with an Argo-LM slide (Argolight, Pessac).

Source link: https://www.degruyter.com/view/j/cdbme.2017.3.issue-2/cdbme-2017-0141/cdbme-2017-0141.xml

Keywords: Intensity response linearity

In-focal-plane characterization of excitation distribution for quantitative fluorescence microscopy applications

Reference: K. Dietrich, M. Brülisauer, E. Çağına, D Bertscha, S Lüthi, P. Heeba, U. Stärkerb, and A. Bernard, “In-focal-plane characterization of excitation distribution for quantitative fluorescence microscopy applications,” DOI: 10.1117/12.2270316, Proceedings of SPIE Optical Metrology, Munich, Germany (June 2017).

Affiliation:
– Institute for Micro and Nanotechnology, Interstaatliche Hochschule für Technik, NTB (Buchs, Switzerland)
– Volpi AG (Schlieren, Switzerland)

They mentioned us with the framework of a study presenting a method to quantitatively characterize novel reference materials as a calibration reference for biomaterials analytics. The investigated materials are thin layers of fluorophores embedded in polymer matrices.

Citation page 2:

Recently, there are also glass slides with laser written, buried fluorescent patterns available.

Source link: https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10333/103330L/In-focal-plane-characterization-of-excitation-distribution-for-quantitative-fluorescence/10.1117/12.2270316.short?SSO=1

Keywords: Quantitative fluorescence microscopy

Reproducibility in light microscopy: Maintenance, standards and SOPs

Reference: R. C. Deagle, T.-L. Wee, C. M. Brown, “Reproducibility in light microscopy: Maintenance, standards and SOPs,” DOI: 10.1016/j.biocel.2017.06.008, International Journal of Biochemistry and Cell Biology 89, 120-124 (June 2017).

Affiliation:
– Advanced BioImaging Facility (ABIF), McGill University (Montreal, Canada)
– Department of Physiology, McGill University (Montreal, Canada)

They mentioned us for lateral aberration assessment within the framework of maintenance and calibration testing of fluorescence microscopes.

Citation page 122:

An expensive, however very effective standard, is the Argolight calibration slide which does not deteriorate or photo-bleach, and its design is platform independent and optimal for lateral aberration assessment.

Source link: https://www.sciencedirect.com/science/article/pii/S1357272517301449?via%3Dihub

Keywords: Lateral aberration

i

Patents mentioning Argolight

Here some patents who are mentioning Argolight and our products.

Oblique plane microscopy system and method
Product: Argo-SIM

Reference: A. York and A. Millett-Sikking, “Oblique plane microscopy system and method,” WIPO Patent Application WO/2020/176591, PCT/US2020/019847 (September 2020)

Affiliation:
– Calico Life Sciences LLC (San Francisco, USA)

They mentioned us for performance validation of a high numerical aperture light-sheet oblique plane microscope that uses only one objective at the sample.

Citation page 27 and 29:

The system was used to image an Argolight SIM slide with a 488 nm laser excitation in a simple epi configuration. […] Four different patterns on the Argolight SIM slide were used for imaging: Target: a 240 µm diameter set of concentric rings with a 10 µm spacing. Each ring consisted of two line pairs separated by 750 nm (good for looking at the maximum field of view); Grid: a 110×110 µm2 square grid with 10 µm spacing. Each line pair is separated by 750 nm (good for looking at the central higher-quality portion of the field of view and checking for field flatness and distortion); SIM lines: 14 line pairs that range from fully overlapped to a separation of 390 nm in 30 nm steps (good for evaluating resolution); 3D rings: 9×9×9 3D cubic array of submicron-diameter rings separated by 5 µm in X, Y, and Z. (good for evaluating a 3D field of view).

Source link: https://www.freepatentsonline.com/WO2020176591A1.html

Keywords: Performance validation

Intensity calibration within the framework of increasing reproducibility and mitigating differences in detection efficiency between channels/image intensity calibration module
Reference: K. Garsha and D. Harshman, “System level calibration,” United States Patent Application Publication, US2020/0080940 A1 (March 2020).

Affiliation:
– Ventana Medical Systems, Inc. (Tucson, USA)

They mentioned us for intensity calibration within the framework of increasing reproducibility and mitigating differences in detection efficiency between channels/image intensity calibration module.

Citation:

A calibration sample (e.g. a standardized photoluminescent sample that will not photobleach) can be utilized in this second calibration procedure. In general, calibration samples of this type permit use of the actual filters and mirrors used for imaging in each channel. In some embodiments, the calibration sample has a large homogeneous area to image such that the average intensity of the homogeneous feature over many pixels can be averaged. In some embodiments, the calibration sample is a stable fluorescent standard sample and/or an imaging filter cube. In some embodiments, the stable fluorescent standard is an Argo-M slide available from Argolight (Pessac, France).

Source link: http://www.freepatentsonline.com/y2020/0080940.html

Keywords: Intensity calibration

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