Argo-SIM Slide V2

Fluorescent* calibration slide for structured illumination systems (SIM) designed for the quality control of microscope (QC).

Argo-SIM slides are specifically designed
for structured illumination microscopes,

and suited for algorithm-based super-resolution microscopes.

Example of suited systems

N-SIM line

Elyra line
(Lattice SIM)

LSM Airy Scan

Stellaris Confocal

Get quality and performance assessment solutions
Contact your local product specialist or someone from Argolight for more information.

User stories

Maria Smedh and Julia Fernandez-Rodriguez

Respectively Researcher, and Head of the Centre for Cellular Imaging, Core Facility of the University of Gothenburg

“The intensity of the new slide is ~ 3-5 times brighter, depending on wavelength, compared to the 1st gen. This makes the patterns easier to find and it is easier to measure them in the orange-red and far-red wavelength regions. (…)The imaging direction is labeled on the new slide. This is nice, especially for unexperienced users, since it reduces mistakes.”

Test carried out on Zeiss confocal (LSM 780) with Argo-SIM V2.

Dr. Patrick Then

Microscopy specialist, Microverse Imaging Center, Friedrich Schiller University Jena

“Both on our wide-field and confocal microscopes, it was clear to see that the brightness of the slides has been nicely improved on the new generation of slides. (…) Being a new facility, we are currently setting up some testing protocols, which we intend to do regularly to make sure our microscopes have reliable performances (…) For that, the Argolight slides are a very nice tool, because the measures are reproducible and signal intensity doesn’t change much over time. We’re thus using the slide as a standard testing target at our facility.”

Test carried out on Zeiss Widefield (Elyra 7 Lattice SIM) and ZEISS Confocal (LSM 980) with Argo-SIM V2.

Jeroen Kole

Product manager at Confocal.NL

“The RCM system is able to achieve 120 nm resolution which is hard to quantify using fluorescent beads. The Argo SIM V2 allowed us to visualize and quantify the resolution in a manner without introducing user-bias. We were clearly able to observe the 120 nm spacing between the lines in the image below. I noticed that the V2 version of the Argo SIM is much brighter than the first version. (…) for the first generation I used to push the laser to 50-60%, the increased brightness of the second-generation Argo SIM allowed me to obtain a similar SNR with only 5% laser power! ”

Test carried out on Re-scan Confocal Microscope 2 (RCM2) with Argo-SIM V2.

Argolight slides are designed to do routine quality assessments and reproducibility assessments of light microscopes.

They are made to improve reproducibility of light microscopy image data through quality control management of instruments (QC).

Argo-SIM slides are re-usable long lasting fluorescent slides. While intensity may fluctuate with time, we warranty that they will be fluorescent for a lifetime.

Argo-SIM slides fluorescence excitation spectrum is a continuum. All slides are compatible with any excitation from 250 nm to 650 nm. Emission is also a continuum starting about 10-20 nm after excitation and ending around 800 nm.

Each Argo-SIM slide contains
several fluorescent microscopic patterns.

Each pattern is designed to be processed with the free companion software to detect aberrations and shift in microscopes performance. Users can catch issues before they can impact image quality and/or use the slides images to troubleshoot the image.

Argo-SIM specifications

Lifetime warrantied
fluorescence presence
Excitation range:
continuum 250-650 nm
Storage conditions:
room temperature (10-40 °C)
and under normal relative humidity
(20-70 % RH).
Dimensions:
75x25x1,5 mm
Emission range:
continuum from the excitation
wavelength plus 15 nm,
to 800 nm
Imaging technology
compatibility:

any fluorescence-based imaging
except depletion-based
technology and
multiphoton imaging
Materials:
Anodized aluminum enclosure
with an Ag03 glass core
Immersion medium
compatibility:

dry, oil: no limitation,
water objectives: less than 20 min at a time
Light exposure
damage threshold:

50 GW.Cm-2 irradiance
(peak or average)

Content of the slide

Each Argo-SIM slide contains 27 fluorescent patterns.

2×16 Intensity Gradation

PAT-AG03-EM2-D2
This pattern consists of twice sixteen 15.0 μm × 0.7 μm rectangles having different fluorescence intensity levels following a linear evolution, organized in a 2×16 matrix.

3D Crossing stairs

PAT-AG03-EM2-I5
This pattern consists of twice 11 empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. The step is: 1 μm.

3D Crossing stairs

PAT-AG03-EM2-I6
This pattern consists of twice 11 empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. The step is: 0.5 μm.

3D Crossing stairs

PAT-AG03-EM2-I7
This pattern consists of twice 11 empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. The step is: 0.25 μm.

3D Crossing stairs

PAT-AG03-EM2-I8
This pattern consists of twice 11 empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. The step is: 0.125 μm.

3D Matrix of rings

PAT-AG03-EM2-K1
This pattern consists in a 3D matrix of 9×9×9 rings, separated by 5 μm, on a total volume of 40 µm × 40 µm × 40 μm.

4×4 Intensity Gradation

PAT-AG03-EM2-C2
This pattern consists of sixteen 6 μm-wide squares having different fluorescence intensity levels following a linear evolution, organized in a 4×4 matrix.

Field of Rings on a background

PAT-AG03-EM2-L1
This pattern consists in a matrix of 9×9 rings, separated by 5 μm, on a total field of 40 µm × 40 μm, on a fluorescent background that is 10 μm below.

Field of Rings

PAT-AG03-EM2-B2
This pattern consists of a matrix of 21×21 rings, separated by 5 μm, on a total field of 100 µm × 100 μm. The field of rings is surrounded by eight landmarks and exhibits a 3 μm long cross in its center.

Geometrical Figure Circle

PAT-AG03-EM2-M1
One of the geometrical figures: a circle.

Geometrical Figure Heptagon

PAT-AG03-EM2-M6
One of the geometrical figures: a heptagon.

Geometrical Figure Hexagon

PAT-AG03-EM2-M5
One of the geometrical figures: a hexagon.

Geometrical Figure Hexagon

PAT-AG03-EM2-M6
One of the geometrical figures: a heptagon.

Geometrical Figure Pentagon

PAT-AG03-EM2-M4
One of the geometrical figures: a pentagon.

Geometrical Figure Rectangle

PAT-AG03-EM2-M3
One of the geometrical figures: a square.

Geometrical Figure Start

PAT-AG03-EM2-M8
One of the geometrical figures: a star with 16 arms.

Geometrical Figure Triangle

PAT-AG03-EM2-M2
One of the geometrical figures: a triangle.

Gradually Spaced Lines

PAT-AG03-EM2-E5
This pattern consists of pairs of 36 μm-long lines whose spacing gradually increases, from 0 nm to 390 nm, with a step of 30 nm. One set of lines is present: horizontal.

Gradually Spaced Lines

PAT-AG03-EM2-E6
This pattern consists of pairs of 36 μm-long lines whose spacing gradually increases, from 0 nm to 390 nm, with a step of 30 nm. One set of lines is present: vertical.

Gradually Spaced Lines

PAT-AG03-EM2-E7
This pattern consists of pairs of 36 μm-long lines whose spacing gradually increases, from 0 nm to 390 nm, with a step of 30 nm. One set of lines is present: descending (- 45°).

Gradually Spaced Lines

PAT-AG03-EM2-E8
This pattern consists of pairs of 36 μm-long lines whose spacing gradually increases, from 0 nm to 390 nm, with a step of 30 nm. One set of lines is present: ascending (+ 45°).

Grid

PAT-AG03-EM2-N1
This pattern consists of a grid with a size of 110 µm × 110 μm and a step of 10 μm, containing crosses of 5 μm length in five squares around the center.

Matrix of crosses

PAT-AG03-EM2-F2
This pattern consists of a matrix of 4×4 crosses, having a length of 5 μm and a step of 10 μm, surrounded by a 40 μm-wide frame.
The crosses are composed of vertical lines that are in the same plane, and by horizontal lines, going gradually deeper within the glass.
The spacing between the vertical and horizontal lines gradually increases, from 0 µm to 1.5 μm, with a step of 0.1 μm.

Repositionning Crosses

PAT-AG03-EM2-H2
The repositioning crosses are 20 μm long.

Sphere

PAT-AG03-EM2-G2
This pattern consists of three circles with a diameter of 25 μm in different orthogonal planes, featuring the equator and two meridians of a sphere.

Target

PAT-AG03-EM2-A2
This pattern consists of concentric circles with increasing radii from 10 μm to 120 μm with a step of 10 μm, featuring a target.

Word ARGOLIGHT

PAT-AG03-EM2-J2
This pattern consists of the letters forming the company name “Argolight”, and surrounded by an 80 μm × 18 μm frame.

Argo-SIM Slide V2

Find product documentation

Find the right documentation, user guide or information about your Argolight product.

Want to know more or ask for a quotation