Stability of Argolight Fluorescent Slides

 

Stability on the shelf

Argolight slides do not exhibit any modification in their fluorescence intensity, spectrum shape or pattern spatial dimensions when stored.

Two environmental accelerated tests, one in temperature and another in humidity, have been carried out in order to evidence the stability of Argolight slides:

  • They have been placed in a climatic test chamber for six months at 100°C
  • They have been immerged in distilled water: at ambient temperature (23°C), as well as at 100°C, for durations up to 500 hours.

No modification of the fluorescence emission has been detected when comparing the measurements before, during and after these tests.

Last but not least, the first slides prototypes produced 8 years ago still exhibit important amount of fluorescence.

 

 

 

Light exposure stability

Argolight patterns do not photobleach, but under high exposure they undergo what we call “reversible photoactivation”.

Photobleaching is the destruction of fluorescent emitters that leads to permanent extinction of fluorescence. Argolight fluorescent emitters do not degrade over time and cannot be destroyed by light sources conventionally used in imaging. That is why Argolight slides still emit fluorescence even after several years of use.

Important: While the patterns do not suffer from photobleaching, the glass itself has a damage threshold. This threshold is expressed in peak irradiance: 50 GW.cm-2. If overstepped, the glassy matrix can be damaged and/or the fluorescence erased.

Reversible photoactivation is the term we use to describe the transient decrease of fluorescence undergone by the patterns when exposed to an important light flux. It is a reversible behaviour: as soon as the exposure is interrupted, the patterns start to recover their fluorescence, eventually reaching their initial level of fluorescence.

This behaviour is an exponential decay with at least two main components: a short one (A) and a long one (B), with B >> A.  The value of these components depends on the imaging systems and conditions: power on the slide (both average and peak), excitation wavelength, objective numerical aperture, exposure time, pixel dwell time, pixel size, etc..

exposure time fluorescence graph typical decrease exposure time fluorescence graph typical recovery

Some examples of decrease/recovery:

Type of system Irradiance (W.cm-2) at 470 nm Illumination duration Intensity decrease (%) Recovery time for > 80% Recovery time for > 90% Total recovery time for > 99%
Widefield ~ 10 60 seconds of continuous exposure ~ 5 N/A* N/A* 5 min
Confocal ~ 10 000 60 seconds of continuous scanning ~ 30 10 min A few weeks A few months

From our experience, we have never been able to observe a decrease of the fluorescence intensity higher than 50%.

*Not Applicable

 

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