Download our technical note on phase contrast in addition to fluorescence

Argolight patterns have successfully been imaged with a quantitative phase microscope equipped with a a SID4 BIO camera from Phasics. In addition to fluorescence contrast, Argolight patterns have been shown to exhibit a phase contrast as well, due to a RI difference of about +10-3 (for Argo-HM products) relative to the surrounding glassy matrix. Spatial correlation between the phase contrast and the fluorescence contrast has been observed. This opens applications in correlative fluorescence and phase contrast microscopy: co-registration correction between images acquired in fluorescence and phase contrast modalities, phase contrast microscope quality control, validation of light propagation reconstruction algorithms for 3D imaging, etc.