Argo-HM Slide V2

Fluorescent calibration* slide for high magnification systems designed for the quality control of confocal microscopes (QC).

Argo-HM slides are specifically designed

for high magnification microscopes,

and especially suited for confocal microscopes

Example of suited systems

X / AX-R line

CSU

X-Light line

LSM

Stellaris Confocal

Thunder

Get quality and performance assessment solutions
Contact your local product specialist or someone from Argolight for more information.

User stories

Dr Steffen Dietzel

Head of Core Facility, Ludwig-Maximilians-Universität, Munich

“We could test the new slide: it was indeed much brighter than our old one. In particular with white light laser systems, where each line is rather dim compared to single line lasers, that made a huge difference. We now could record images with 638 nm excitation which was not possible before.”

Test carried out on Leica Confocal (SP8), with Argo-HM V2.

Dr. Martin Spitaler

Light Microscopy Facility Manager, Max Planck Institute of Biochemistry, Martinsried

“We’ve been using our previous Argolight slide to do regular Quality Control on all our microscopes. The new generation is much brighter, in our experience about 2-4x, compared to the old slides. That significantly improves QC with low NA/magnification lenses and in the red channels.”

Test carried out on Leica Widefield (Thunder) and Leica Confocal (SP8) with Argo-HM V2.

Dr Alexia Loynton-Ferrand

Research Associate in Advanced Light Microscopy, Biozentrum Imaging Core Facility, Basel

“The slide is indeed brighter. From the histogram during acquisition, I would say it is double. It makes the acquisition comfortable, and less prone to bleaching. (…) We use the HM slide quite a lot, usually imaging six patterns. Overall, while the first generation of slides was already very nice, the 2nd generation is even more comfortable to use, while keeping all the good features of the previous version.”

Test carried out on Deltavision widefield and ZEISS Confocal (LSM800) with Argo-HM V2.

Argolight slides are designed to do routine quality assessments and reproducibility assessments of light microscopes.

They are made to improve reproducibility of light microscopy image data through quality control management of instruments (QC).

Argo-HM slides are re-usable long lasting fluorescent slides. While intensity may fluctuate with time, we warranty that they will be fluorescent for a lifetime.

Argo-HM slides fluorescence excitation spectrum is a continuum. All slides are compatible with any excitation from 250 nm to 650 nm. Emission is also a continuum starting about 10-20 nm after excitation and ending around 800 nm.

Each Argo-HM slide contains
several fluorescent microscopic patterns

Each pattern is designed to be processed with the free companion software to detect aberrations and shift in microscopes performance. Users can catch issues before they can impact image quality and/or use the slides images to troubleshoot the image.

Argo-HM specifications

Lifetime warrantied
fluorescence presence
Excitation range:
continuum 250-650 nm
Storage conditions:
room temperature (10-40 °C)
and under normal relative humidity
(20-70 % RH).
Dimensions:
75x25x1,5 mm
Emission range:
continuum from the excitation
wavelength plus 15 nm,
to 800 nm
Imaging technology
compatibility:

any fluorescence-based imaging
except depletion-based
technology and
multiphoton imaging
Materials:
Anodized aluminum enclosure
with an Ag03 glass core
Immersion medium
compatibility:

dry, oil: no limitation,
water objectives: less than 20 min at a time
Light exposure
damage threshold:

50 GW.Cm-2 irradiance
(peak or average)

Content of the slide

Each Argo-HM slide contains 16 fluorescent patterns.

2×16 Intensity Gradation

PAT-AG03-EM1-D1
This pattern consists of twice sixteen 22.5 μm × 1.5 μm rectangles having different fluorescence intensity levels following a linear evolution, organized in a 2×16 matrix.

3D Crossing stairs 1um step

PAT-AG03-EM1-I1
This pattern consists of twice 11 empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. The step is: 1 μm.

3D Crossing stairs 250 nm step

PAT-AG03-EM1-I4
This pattern consists of twice 11 empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. The step is: 0.25 μm.

3D Crossing stairs 500 nm step

PAT-AG03-EM1-I3
This pattern consists of twice 11 empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. The step is: 0.5 μm.

3D Crossing stairs 750 nm step

PAT-AG03-EM1-I2
This pattern consists of twice 11 empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. The step is: 0.75 μm.

4×4 Intensity Gradation

PAT-AG03-EM1-C1
This pattern consists of sixteen 8.5 μm-wide squares having different fluorescence intensity levels following a linear evolution, organized in a 4×4 matrix.

Field of rings

PAT-AG03-EM1-B1
This pattern consists of a matrix of 45×45 rings, separated by 15 μm, on a total field of 660 µm × 660 μm. The field of rings is surrounded by eight landmarks and exhibits an 8 μm long cross in its center.

Gradually Spaced Line Descinding

PAT-AG03-EM1-E4
This pattern consists of pairs of 50 μm-long lines whose spacing gradually increases, from 100 nm to 700 nm, with a step of 50 nm. One set of lines is present: ascending (+ 45°).

Gradually Spaced Line Descinding

PAT-AG03-EM1-E3
This pattern consists of pairs of 50 μm-long lines whose spacing gradually increases, from 100 nm to 700 nm, with a step of 50 nm. One set of lines is present: descending (- 45°).

Gradually Spaced Line Horizontal

PAT-AG03-EM1-E1
This pattern consists of pairs of 50 μm-long lines whose spacing gradually increases, from 100 nm to 700 nm, with a step of 50 nm. One set of lines is present: horizontal.

Gradually Spaced Line Vertical

PAT-AG03-EM1-E2
This pattern consists of pairs of 50 μm-long lines whose spacing gradually increases, from 100 nm to 700 nm, with a step of 50 nm. One set of lines is present: vertical.

Matrix of Crosses

PAT-AG03-EM1-F1
This pattern consists of a matrix of 6×6 crosses, having a length of 5 μm and a step of 8 μm, surrounded by a 50 μm-wide frame.
The crosses are composed of vertical lines that are in the same plane, and by horizontal lines, going gradually deeper within the glass.
The spacing between the vertical and horizontal lines gradually increases, from 0 µm to 3.5 μm, with a step of 0.1 μm.

Repositionning Crosses

PAT-AG03-EM1-H1
The repositioning crosses are 40 μm long.

Sphere

PAT-AG03-EM1-G1
This pattern consists of three circles with a diameter of 50 μm in different orthogonal planes, featuring the equator and two meridians of a sphere.

Target

PAT-AG03-EM1-A1
This pattern consists of concentric circles with increasing radii from 25 μm to 325 μm with a step of 25 μm, plus an extra circle with a radius of 12.5 μm, featuring a target.

Word ARGOLIGHT

PAT-AG03-EM1-J1
This pattern consists of the letters forming the company name “Argolight”, and surrounded by a 220 μm × 50 μm frame.

Argo-HM Slide V2

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