Welcome to Daybook 2
Get stellar data on your microscope performances!
HOW IT WORKS
IMAGE THE PRODUCT
Image the pattern(s) of interest on your Argolight product
Run your image analysis: several automatized tests and tools
EXPLORE & REPORT
Visualize data, monitor results and manage reports
Analyse and perform multi tests (resolution, inhomogeneity, chromatic shifts…) on multiformats images (.czi, .nd2, .lif, .scn, .oif…)
Get factual and comparable results and data
DAYBOOK DATA MANAGER
Follow and manage several microscopes with different configurations.
Explore, report and share specific data of your different profiles.
Daybook Analysis module allows to perform a large range of tests on your microscope. Here is what you can measure: learn more about our products.
Line spread function
Stage drift during Z stacking
Lateral co-registration accuracy
Stage motion control
Field uniformity Test
In any fluorescence microscope, the spatial distribution of the illumination intensity is never constant. It is usually maximal in the center of the field of view (if the microscope is well-aligned), and decreases towards the edges, so that a biological sample is never homogeneously illuminated.
For information, other appellations than homogeneity can be found in the literature: uniformity, evenness, flatness of illumination, or shading. Here our approach is to use a matrix of fluorescent rings.
Lateral Resolution Test
The lateral resolution test is defined as the shortest distance between two lines on a fluorescent pattern that can still be distinguished by the observer or camera system as separate entities.
In any fluorescence microscope, a degradation of the lateral resolving power induces a loss of contrast in sample images. Many aspects can modify the system’s PSF and therefore its lateral resolving power.
Field Distortion Test
Field distortion is an optical aberration inducing image deformation, usually at the corners of the image. In the presence of distortion, the magnification is not constant over the FOV; it is dependent on the (x ; y) coordinates. In any fluorescence microscope, the knowledge of the field of view distortion is important when spatial information in an image, such as distances, is aimed to be measured.
Distortion results in spatial variation of the pixel size, i.e. the value of the pixel size depends on its location in the field of view. The distortion rate (DR) of microscope objectives is not provided by the manufacturers.
Intensity Response Test
The overall intensity response of fluorescence microscopes may evolve over time, especially on confocal microscopes, and widefield microscopes equipped with metal-halide illuminators. In order to check the linearity of the intensity response we are using sixteen squares having different fluorescence intensity levels following a linear evolution.
Line Spread Function Test
The Line Spread Function test provides information on how and how much the light spreads from a line. The intensity spreading from a line can be modelled with a Gaussian function, which full-width at half maximum (FWHM) informs on the spreading behavior.
The cross inside the field of rings can be used to extract the line spread function in both the vertical and horizontal directions.
Stage Drift During Z Stacking Test
The environmental conditions around a fluorescence microscope can influence its performances. This is even more true if high resolution and/or precision are required for a given experiment. In particular, the lateral stage drift during Z-stack imaging is important when temperature and air flow fluctuate.
Spectral Response Test
Fluorescence microscopes, although conceived preliminary to be imaging systems, can also acquire emission spectra, with a better spatial resolution but poorer spectral resolution than conventional spectrometers.
Correct interpretation of the emission spectra measured with a microscope requires the knowledge of its spectral response.
Lateral co-registration accuracy Test
In any fluorescence microscope, the knowledge of the chromatic shifts, both in the lateral and axial directions, is important when color information in an image is aimed to be used. In particular, for co-localization tests in images of biological samples stained with several labels, chromatic shifts between the different channels must be known and compensated to prevent from misinterpretation.
Stage Drift During Timelapse
When performing timelapse imaging, it is important to be sure that the lateral stage do not drift too much. This test allows to measure the lateral stage drift during a timelapse imaging, to ensure it is not too important for the application.
Stage Repositioning Repeatability Test
Stage repositioning repeatability is of importance for stitching imaging. This test allows to determine the stage repositioning repeatability after many round trips, to ensure if it is within the manufacturer specifications.